Emerging Technologies in probiotic, live biotherapeutic product and microbiome analysis

Emerging Technologies in probiotic, live biotherapeutic product and microbiome analysis

Agenda | Speaker Biographies | Event Summary (Coming Soon) | Event Presentations

The United States Pharmacopeia held a free, virtual scientific symposium, “Emerging technologies in probiotic, live biotherapeutic products and microbiome analysis,” on October 6-7, 2022, 8:30-11:30 AM US Eastern Time.

The goals of the symposium included:

  • Highlighting the newer technologies for identification and enumeration of live bacteria, such as qPCR, ddPCR and flow cytometry
  • Understanding the theory behind these technologies and their performance, and
  • Understanding current and possible future uses of these technologies. Case studies for each were also presented. Symposium attendees learned about these technologies from experts in the field, including how to implement them and the advantages and limitations of each technology.

Attendees included

Professionals from contract testing laboratories, as well as from probiotic, live biotherapeutic product, and microbiome industries, including researchers, product manufacturers, and quality assurance

Event Presentations

Day One

  1. Opening Remarks, USP Overview, Kit Goldman, Ph.D., | USP
  2. Challenges and Opportunities in the Analysis of Live Microorganisms, Jean Schoeni, Ph.D., | USP
  3. Quantitative Real-Time PCR for the Determination of Probiotic Potency, Andrzej Benkowski, B.Sc., | Eurofins
  4. Advanced Molecular Tools for the Analysis of Beneficial Microbes in Foods, Mickaël Boyer, Ph.D., | Lesaffre International
  5. Absolute Quantification of Organisms by Droplet Digital PCR (ddPCR) – An Overview of the Technology and Method, Michael Geimer, B.S., | ATCC
  6. Droplet Digital PCRF (ddPCR) Case Study, Anthony Kiefer, | International Flavors & Fragrances
  7. Challenges and opportunities of DNA-based identification, characterization, and authentication of probiotic strains, Antonio Del Casale, CEO | Microbion SRL
  8. NextGen Sequencing and Metagenomics, Scott Jackson, | National Institute of Standards and Technology

Day Two

  1. Intro to Flow Cytometry Antibody Identification, Pierre Burguière, Ph.D. | USP
  2. Label-free Enumeration of Bacteria by Impedance Flow Cytometry, Peter Lüttge Jordal, Ph.D. ǀ SBT Instruments A/S
  3. Assessment of Long-Term Shelf-Life (and Metabolic Viability) of a Multi-strain Synbiotic Using Standard and Innovative Enumeration Methodologies, Marco Pane, Ph.D. ǀ Probiotical Research srl
  4. Rapid Monitoring of Microbiota Composition and Targeted Culturomics of Species of Interest using Polyclonal Antibodies in Combination with Flow Cytometry, Vincent Thomas, Pharm.D., Ph.D. | Bioaster
  5. Thermocalometric Cell Counting, Marta Veses Garcia, Ph.D. ǀ Symcel