Course Description:
The primary method for quantitation and characterization of synthetic oligonucleotides is ion-pair reversed phase liquid chromatography (IP-RPLC). Mobile phase composition is typically an
alkylamine such as triethylamine (TEA) and perfluorinated alcohol such as hexafluoroisopropanol (HFIP), with methanol as the strong solvent. In this presentation, we will demonstrate the impact of
mobile phase optimization on chromatographic separation and MS sensitivity and spectral quality. Additionally, we will look into how temperature and flow-rate can impact oligo separations.
The live version of this recording took place on March 3, 2021 and features
a presentation by Brian Rivera
Who should participate:
- Analytical chemists
- QA/QC analysts
- R&D scientists, managers
- Team members in CMC development projects
- Manufacturing scientists, managers
- Regulatory affairs specialists
- Contract research organizations
- Contract manufacturing organizations
Access Duration:Access to this course expires 60 days from the date of registration or until you mark the course ‘Complete’ – whichever occurs first.